Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C43 (DE3) cells for full-length protein and BL21 (DE3) pLysS (DE3) for the CBS domain | Archaeoglobus fulgidus |
Crystallization (Comment) | Organism |
---|---|
regulatory CBS domain pair of the enzyme in the apo and nucleotide-bound form, sitting drop vapor diffusion method, using 0.1 M Bis-Tris pH 6.5, 0.2 M (NH4)2SO4, 20% (w/v) PEG 3350 | Archaeoglobus fulgidus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Archaeoglobus fulgidus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains zinc | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O29915 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Strep-Tactin column chromatography, and Superdex S200 gel filtration | Archaeoglobus fulgidus |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
MEROPS:M50 | - |
Archaeoglobus fulgidus |
S2P | - |
Archaeoglobus fulgidus |
site-2 protease | - |
Archaeoglobus fulgidus |